Olympus BX60 Epifluorescence Microscope

The PIC has a conventional epifluroescence microscope made by Olympus. It features three cubes (UV, blue and green excitation - see specifications below), two types of arc lamps (mercury or xenon), a sutter excitation filter wheel with shutter, and two digital cameras (a color Olympus DP70 and a Photometrix CCD). This microscope is useful for fluorescence imaging of larger samples (the lowest power objective is a 2.5x) and for screening for fluorescence. In addition, the instrument can be configured for calcium ratio imaging using Fura. Excellent bright field digital images are also possible to capture using this equipment.

 

Filter Cubes, PIC Olympus Epifluorescence Microscope

Position "WU"
U-MWU
Olympus UV excitation
ex 330-385
bs 400
em 420 LP

Position "A"
41020 HQ NB GFP C-53502
Chroma Narrow-band GFP, for minimizing autofluorescence.
ex 480/20
bs 495
em 510/20

Position "WIG"
U-MWIG
Olympus Rhodamine
ex 520-550
bs 565
em 580

Position "NIB"
U-MNIB
Olympus FITC
ex 470-490
bs 505
em 515 LP

 

Two extra cubes not installed:
U-M 61002 403 "DAPI/FITC/TXSRED"
Fura2 (NOTE: NO EX FILTER! Use with filter wheel.) Chroma 71000A C71135

Olympus cube specifications.