Zeiss Elyra PS.1 System

For super-resolution, the inverted microscope has four laser lines (405, 488, 561 and 640nm) and five color filtersets for both PAL-M and SR-SIM super-resolution imaging. It is equipped with two EM-CCD cameras, an Andor iXon3 897 camera for PAL-M imaging in TIRF mode and an Andor iXon3 885 camera for 3D SR-SIM.

For confocal, six laser lines are available (405, 458, 488, 514, 561, and 633nm). It is also equipped with a 34 element internal GAsP detector (LSM 780) for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging.

Microscope optics include the 100x/1.46 alpha Plan-APO for TIRF, the Plan-Apochromat 63x/1.40 for SIM, and 100x/1.57 alpha Plan-APO for PALM, 40x/1.10 C-Apochromat for confocal, and adapters for our current Zeiss objectives. Filter sets include a 4 laser line primary beamsplitter for optimal unmixing capability.

Additional capabilities are a joystick-controlled motorized stage, heated stage insert, and a fiber optic-coupled computer running Zeiss’ ZEN software capable of imaging the same sample using three different technologies in succession (confocal, SR-SIM and PAL-M).

Zeiss Elyra

The Zeiss Super-Resolution Elyra PS.1 System.